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α fetoprotein afp mouse anti human monoclonal antibody  (R&D Systems)


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    Structured Review

    R&D Systems α fetoprotein afp mouse anti human monoclonal antibody
    α Fetoprotein Afp Mouse Anti Human Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+afp/Human%2FMouse+alpha-Fetoprotein%2FAFP+Antibody/pm40496982-55-39-48
    Average 93 stars, based on 128 article reviews
    α fetoprotein afp mouse anti human monoclonal antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting Wnt and BMP signaling
    Article Snippet: Lysates were boiled for 5 min in Laemmli sample buffer and 10-40 μg protein was separated by electrophoresis on 10% or 4- 15% Mini-PROTEAN TGX gels (Bio-Rad) or NuPage 4-12%, BisTris SDS-PAGE gels in MOPS buffer (ThermoFisher) and transferred to nitrocellulose (Amersham Protran) or PVDF (Bio-Rad) membranes using the Bio-Rad Mini-Protean transfer system. .. Membranes were blocked in 5% skimmed milk in PBS or SuperBlock (ThermoFisher) for 1 h at RT and incubated overnight at 4°C with the following primary antibodies: goat polyclonal anti-PDX1 (R&D Systems # AF2419) 1:500; goat polyclonal anti-OTX2 (R&D Systems # AF1979) 1:1,000; rabbit polyclonal anti-AFP (DAKO/Agilent # A0008) 1:500; mouse monoclonal anti-AFP (R&D Systems # MAB1368) 1:1,000; mouse monoclonal anti-b-catenin (BD Biosciences # 610154) 1:2,000; mouse monoclonal anti-vinculin (Sigma-Aldrich # V9131) 1:5,000 and 1:10,000 and rat monoclonal anti-tubulin (Abcam # ab6160) 1:2,000. .. After three washes with TBST (0.1% Tween-20 in 1x Tris-buffered saline) or PBST (0.1% Tween-20 in 1x PBS), the blot was incubated with respective secondary HRP- or Alexa Fluor-conjugated antibodies at RT for 30 or 60 min. ECL Prime Western Blotting Detection Reagent was used for detection according to the manufacturer’s instructions (Sigma-Aldrich).

    Article Title: TGF-β modulates cell fate in human ES cell-derived foregut endoderm by inhibiting Wnt and BMP signaling
    Article Snippet: Lysates were boiled for 5 min in Laemmli sample buffer and 10-40 μg protein was separated by electrophoresis on 10% or 4- 15% Mini-PROTEAN TGX gels (Bio-Rad) or NuPage 4-12%, BisTris SDS-PAGE gels in MOPS buffer (ThermoFisher) and transferred to nitrocellulose (Amersham Protran) or PVDF (Bio-Rad) membranes using the Bio-Rad Mini-Protean transfer system. .. Membranes were blocked in 5% skimmed milk in PBS or SuperBlock (ThermoFisher) for 1 h at RT and incubated overnight at 4°C with the following primary antibodies: goat polyclonal anti-PDX1 (R&D Systems # AF2419) 1:500; goat polyclonal anti-OTX2 (R&D Systems # AF1979) 1:1,000; rabbit polyclonal anti-AFP (DAKO/Agilent # A0008) 1:500; mouse monoclonal anti-AFP (R&D Systems # MAB1368) 1:1,000; mouse monoclonal anti-b-catenin (BD Biosciences # 610154) 1:2,000; mouse monoclonal anti-vinculin (Sigma-Aldrich # V9131) 1:5,000 and 1:10,000 and rat monoclonal anti-tubulin (Abcam # ab6160) 1:2,000. .. After three washes with TBST (0.1% Tween-20 in 1x Tris-buffered saline) or PBST (0.1% Tween-20 in 1x PBS), the blot was incubated with respective secondary HRP- or Alexa Fluor-conjugated antibodies at RT for 30 or 60 min. ECL Prime Western Blotting Detection Reagent was used for detection according to the manufacturer’s instructions (Sigma-Aldrich).

    other:

    Article Title: Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.
    Article Snippet: The cells were harvested using CTK solution (ReproCELL) and transferred cell clumps onto an ultra-low binding plate (Corning) in EB media (DMEM/F12 containing 20% KSR, 1% GlutaMax, 1% NEAA and 1 3 10 4 M 2-ME).



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    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Terminal α1,2-fucosylation of glycosphingolipids by FUT1 is a key regulator in early cell-fate decisions

    doi: 10.1038/s44319-024-00243-1

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: α-Fetoprotein (αFP) , R&D Systems (mouse, monoclonal) (FACS) , , MAB1368.

    Techniques: Immunohistochemistry-Frozen IF, Western Blot, Recombinant, Staining, Plasmid Preparation, Sequencing, Control, Transfection, Over Expression, Software, TaqMan Assay, Expressing, Binding Assay, Marker